A Brief Review of How to Construct an Enzyme-Based H<sub>2</sub>O<sub>2</sub> Sensor Involved in Nanomaterials

This article briefly reviews how to construct an enzyme based hydrogen peroxide sensor involving nanomaterials, which has the advantages of high efficiency, good sensitivity and selectivity, fast response time extended range linearity with lower detection limit. Glucose biosensor is constructed by immobilizing glucose oxidase on polycarbonate membrane protective cover then filled a physiological phosphate buffer, pH 7.4. The novel blocking hydrophobic only permeable used eliminate electrochemical interferences. H2O2 miniaturized involvement nanomaterials like carbon nanotubes, platinum nanoparticles silver it can achieve effective microscopic glucose. introduction including some pure metals (Ag, Au, Pd, Ni, Pt, Cu), metal oxide (ZnO TiO2), bimetallic (Au/Ag Au/Pt) (nanotubes graphene) in construction improves its performance enhancing enzymatic activity, allows many new signal transduction technologies biosensors. review summarizes working principles sensor, importance manufacturing, basic characteristics components biosensor, generations biosensors, procedure making synthesis involved finally examples intervene biosensor.